QCIF training course: Long-Read Assembly using Galaxy
Register now for this great tutorial run by members of QCIF and GIH's Valentine Murigneux!
This tutorial explores how long and short read data can be combined to produce a high-quality ‘finished’ bacterial genome sequence. Termed ‘hybrid assembly’, we will use read data produced from two different sequencing platforms, Illumina (short read) and Oxford Nanopore Technologies (long read), to carry out de novo genome assembly.
The workshop will focus on the use of Galaxy, a platform that provides a simple and user-friendly interface to bioinformatics tools.
Recommended Participants
Biologists and other life scientists planning to work with long-read (ONT) and Illumina data for genome assemblies. No prior bioinformatics knowledge is required.
Learning Objectives
- Understand how Nanopore and Illumina reads can be used together to produce a high quality assembly
- Be familiar with genome assembly and polishing programs
- Learn how to assess the quality of a genome assembly, regardless of whether a reference genome is present or absent
- Be able to assemble an unknown, previously undocumented genome to high-quality using Nanopore and Illumina reads
For registration details, please follow the link below:
https://www.qcif.edu.au/trainingcourses/hybrid-assembly-galaxy/
Learn how to create and assess genome assemblies using the powerful combination of Nanopore and Illumina reads
About this event
This tutorial explores how long and short read data can be combined to produce a high-quality ‘finished’ bacterial genome sequence. Termed ‘hybrid assembly’, we will use read data produced from two different sequencing platforms, Illumina (short read) and Oxford Nanopore Technologies (long read), to carry out de novo genome assembly.
The workshop will focus on the use of Galaxy, a platform that provides a simple and user-friendly interface to bioinformatics tools.
FAQs
What should I bring into the event?
This is an online workshop, so you will need to provide your own computer and have access to a reliable internet connection sufficient for video conferencing. A second monitor is highly recommended.
What prior experience do I need?
None - the workshop gives a full introduction to both hybrid genome assembly and the Galaxy bioinformatics platform
Who should attend?
The workshop is designed for biologists, other life scientists, and computer scientists planning to work with long-read (ONT) and Illumina data for genome assemblies
How can I contact the organiser with any questions?
You can email training@qcif.edu.au
What's the cancellation policy?
Cancellations are accepted up to 48h before the start of the workshop. If you do not join on the day without having cancelled beforehand, you may be blocked from attending any future QCIF training workshops.