Single-cell and short read sequencing

CAGE (cap analysis gene expression) is highly sensitive and accurate quantitative transcriptome analysis which utilizes a cap structure on 5’ end of RNA. This feature enables high-throughput identification of transcription start sites and ability to infer promoters and transcription factor binding motifs. This project established the availability of CAGE through development of an optimized, cost -efficient in-house protocol, benchmarked against a commercially available ‘gold standard’ kit. This protocol produces comparable results to the commercial kit while introducing savings in reagent costs and time.

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