Research
Long read sequencing
The Genome Innovation Hub continually develops a number of protocols and procedures while undertaking our collaborative projects. Below are a selection of detailed developments useful to researchers undertaking long read sequencing procedures in the laboratory.
Developments
Featured projects | Duration |
---|---|
Culture-independent metagenomic diagnostics Genome Innovation Hub Collaborative Project (UQ infrastructure) |
2021 |
Deciphering the epitranscriptome machinery using direct native RNA sequencing Genome Innovation Hub Collaborative Project (UQ infrastructure) |
2021 |
Assessing DNA Replication Dynamics in Cancer Genome Innovation Hub Collaborative Project (UQ infrastructure) |
2021 |
A genomic dissection of metaorganisms: molecular approaches for teasing apart the hologenome Genome Innovation Hub Collaborative Project (UQ infrastructure) |
2020 |
Targeted capture and detection of 16s rRNA gene applying PCR free long read sequencing Genome Innovation Hub Collaborative Project (UQ infrastructure) |
2020 |
Applying long-read sequencing technology to understand telomere repair and DNA replication Genome Innovation Hub Collaborative Project (UQ infrastructure) |
2019 |
Assembly of complex genome (Macadamia) using single molecule sequencing Genome Innovation Hub Collaborative Project (UQ infrastructure) |
2019 |
Publications
Comparison of long-read methods for sequencing and assembly of a plant genome
Valentine Murigneux, Subash Kumar Rai, Agnelo Furtado, Timothy J C Bruxner, Wei Tian, Ivon Harliwong, Hanmin Wei, Bicheng Yang, Qianyu Ye, Ellis Anderson, Qing Mao, Radoje Drmanac, Ou Wang, Brock A Peters, Mengyang Xu, Pei Wu, Bruce Topp, Lachlan J M Coin, Robert J Henry
GigaScience, Volume 9, Issue 12, December 2020, giaa146, https://doi.org/10.1093/gigascience/giaa146
Improvements in the Sequencing and Assembly of Plant Genomes
Priyanka Sharma, Othman Aldossary, Bader Alsubaie, Ibrahim Al-Mssallem, Onkar Nath, Neena Mitter, Gabriel Margarido, Bruce Topp, Valentine Murigneux, Ardy Masouleh, Agnelo Furtado, Robert J Henry
bioRxiv 2021.01.22.427724; doi: https://doi.org/10.1101/2021.01.22.427724
Webinars
Published on: 1 May 2020
This webinar will present a comparison of three long read sequencing technologies applied to the de novo assembly of a plant genome, Macadamia jansenii, presented by Valentine Murigneux (GIH/QFAB).
GIH Collaborative Project.
Hosted by Australian BioCommons.
Published on: 16 April 2020
In this webinar, Professor Lachlan Coin will talk about his recent study with the Genome Innovation Hub at The University of Queensland for de novo assembly of the genome of Australia’s vulnerable plant species, Macadamia jansenii. In this study several long read sequencing technologies, including MGI’s unique single-tube Long Fragment Read (stLFR), are compared for the same sample.
GIH Collaborative Project.
Hosted by MGI