Abstract representation of a network of start pointsAims

This project aims to introduce the availability of (Cap Analysis Gene Expression (CAGE) through development of an optimized, cost-efficient in-house protocol, benchmarked against a commercially-available ‘gold standard’ kit.

Brief project outline

CAGE is highly sensitive and accurate quantitative transcriptome analysis which utilizes a cap structure on 5’ end of RNA. This feature enables high-throughput identification of transcription start sites and ability to infer promoters and transcription factor binding motifs.

Genomics-based innovative aspect

To our knowledge this is the first successful implementation of CAGE in Australia.

Broad applicability of the technique

Our aim was to introduce a more cost-effective "in-house" version of CAGE available for all UQ researchers to access within UQ sequencing facilities.

Developments arising from this project

GIH Development: Cap Analysis of Gene Expression (CAGE)

 

Project members


Research Collaborators

Joanna Crawford

Joanna Crawford

Senior Research Assistant
Institute for Molecular Bioscience
Dr Quan Nguyen

Dr Quan Nguyen

Senior Research Fellow - Group Leader
Institute for Molecular Bioscience

Genome Innovation Hub

Stacey Andersen

Stacey Andersen

Senior Research Assistant
Genome Innovation Hub
Senior Research Assistant
IMB Sequencing Facility
Dr Sohye Yoon

Dr Sohye Yoon

Research Assistant
Genome innovation Hub
Professor Grant Montgomery

Professor Grant Montgomery

Director
Genome Innovation Hub (GIH)
Professorial Research Fellow - GL
Institute for Molecular Biosciences (IMB)
Affiliate Professorial Research Fellow
Queensland Brain Institute (QBI)
Affiliate Professorial Research Fellow
Office of the Pro-Vice-Chancellor (Research Infrastructure)