In brief

CAGE (cap analysis gene expression) is highly sensitive and accurate quantitative transcriptome analysis which utilizes a cap structure on 5’ end of RNA. This feature enables high-throughput identification of transcription start sites and ability to infer promoters and transcription factor binding motifs. This project established the availability of CAGE through development of an optimized, cost -efficient in-house protocol, benchmarked against a commercially available ‘gold standard’ kit. This protocol produces comparable results to the commercial kit while introducing savings in reagent costs and time.

Highlights

  • CAGE can be used to simultaneously locate transcription start sites and quantify the abundance of RNA transcripts.

  • This developed "In-house" CAGE protocol delivers high quality results at a ~1/3 of the reagent cost and 25% less hands-on time when compared to the commercial kit.

  • To our knowledge this is the first successful implementation of CAGE in Australia.

Standard operating procedure (SOP)

UQ-GIH: inhouseCAGE (PDF, 653.1 KB)

 

GIH team

Dr Sohye Yoon
Dr Sohye Yoon

 

Stacey Andersen
Stacey Andersen

Collaborating researchers

Dr Quan Nguyen
Dr Quan Nguyen