CRISPR-Cas9 and RNAIn brief

CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) technologies enable scientists to accurately modify the genome of cells and organisms. CRISPR-Cas9 and CRISPR-Cas12a enzymes are the most widely studied of the RNA-guided endonucleases used for genome editing. To help reduce costs of our genome editing research projects, we have established an "in-house" protocol for purifiying and functionally testing a range of Cas9/Cas12a endonuclease which are attached to various affinity tags.

Highlights

  • "In-house" purified CRISPR-Cas9 and CRISPR-Cas12a endonuclease can help to reduce laboratory costs of genome editing research.
  • "In-house" purified CRISPR-Cas9 and CRISPR-Cas12a endonuclease offers flexibility to research labs who may want to produce uniquely modified/tagged proteins which are not commercially available.
  • "In-house" purified Cas9 and Cas12a show comparable quality with commercial available products.

 

Standard operating procedure (SOP)

Please contact Stacey Andersen (operations manager) for the updated SOP. 

 

GIH team

Dr Jun Ma
Dr Jun Ma
Dr Di Xia
Dr Di Xia

 

Collaborators

Dr Nathan Palpant
Dr Nathan Palpant