CRISPR-Cas9 and RNAIn brief

CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) technologies enable scientists to accurately modify the genome of cells and organisms. CRISPR-Cas9 and CRISPR-Cas12a enzymes are the most widely studied of the RNA-guided endonucleases used for genome editing. To help reduce costs of our genome editing research projects, we have established an "in-house" protocol for purifiying and functionally testing a range of Cas9/Cas12a endonuclease which are attached to various affinity tags.

Highlights

  • "In-house" purified CRISPR-Cas9 and CRISPR-Cas12a endonuclease can help to reduce laboratory costs of genome editing research.
  • "In-house" purified CRISPR-Cas9 and CRISPR-Cas12a endonuclease offers flexibility to research labs who may want to produce uniquely modified/tagged proteins which are not commercially available.
  • "In-house" purified Cas9 and Cas12a show comparable quality with commercial available products.