Development: Cap analysis gene expression (CAGE)

10 Aug 2020

Cap analysis gene expression (CAGE) is highly sensitive and accurate quantitative transcriptome analysis which utilizes a cap structure on 5’ end of RNA. This feature enables high-throughput identification of transcription start sites and ability to infer promoters and transcription factor binding motifs. This project established the availability of CAGE through development of an optimized, cost -efficient in-house protocol, benchmarked against a commercially available ‘gold standard’ kit. This protocol produces comparable results to the commercial kit while introducing savings in reagent costs and time.

To read the full GIH protocol, see Cap Analysis of Gene Expression in the GIH Developments page.

To enquire about submitting your samples for CAGE as a sequencing service, contact