Expertise:

  • Method development for DNA and RNA sample preparation
  • Oxford Nanopore Sequencing technologies
  • DNA- and RNA-seq data analysis

Researcher biography

Subash completed a BSc in Biotechnology and Genetic Engineering in Bangladesh in 2007. He returned to Nepal and worked as a research technician for 2 years to establish a plant tissue culture lab. With a NFP scholarship, he completed his MSc in Biotechnology from Wageningen University, the Netherlands. After completing his MSc, he moved to Germany and joined a biotechnology company Riboxx GmbH as an Early Stage Researcher under the Marie Curie initial training network and obtained his industrial doctorate (dr.rer.medic) in virology from Technische Universität Dresden in 2015. His work characterising viral proteins and RNA regulatory elements resulted in the identification of two potential antiviral drug-targets for pre-clinical antiviral drug development studies. Before joining GIH in 2018, he worked with Prof. Richard Lewis at IMB where his work emphasised cone snails venom genomics and transcriptomics. Since then, he has worked on multiple projects focusing on various aspects of the long read technologies – sample preparation methods development, sequencing instrumentation, data analysis and training to tune optimal and innovative use of the long read technologies.

Featured projects Duration
Discovery of new regulatory networks with long-read transcriptomics
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2019
Adaptive sequencing for metagenomic sequencing of low biomass samples
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2022
Applying long-read sequencing technology to understand telomere repair and DNA replication
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2019
Identification and exploitation of genome landing pads for cattle.
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2022
Assembly of complex genome (Macadamia) using single molecule sequencing
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2019
A genomic dissection of metaorganisms: molecular approaches for teasing apart the hologenome
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2020
Targeted capture and detection of 16s rRNA gene applying PCR free long read sequencing
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2020
Culture-independent metagenomic diagnostics
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2021
Genome-Phaser: protocol for fully phasing whole genome variants using haplotagging
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2021
Deciphering the epitranscriptome machinery using direct native RNA sequencing
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2021
Assessing DNA Replication Dynamics in Cancer
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2021
Using nanopore sequencing to test mRNA vaccine quality.
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2022
Multi-contact Pore-C
Genome Innovation Hub Collaborative Project (UQ infrastructure)
2022

Areas of research