Publication: Comparison of long read methods

21 Dec 2020

Full Title

Comparison of long-read methods for sequencing and assembly of a plant genome.

Valentine MurigneuxSubash Kumar Rai, Agnelo Furtado, Timothy J C Bruxner, Wei Tian, Ivon Harliwong, Hanmin Wei, Bicheng Yang, Qianyu Ye, Ellis Anderson, Qing Mao, Radoje Drmanac, Ou Wang, Brock A Peters, Mengyang Xu, Pei Wu, Bruce Topp, Lachlan J M Coin, Robert J Henry

GigaScience, Volume 9, Issue 12, December 2020, giaa146,


Benchmarking methods for sequencing complex genomes: how to select the most cost-effective sequencing technology as well as the most appropriate software for assembly and polishing.

In a worlds first, the Genome Innovation Hub and colleagues completed the de novo assembly of the genome of the Australiian wild Macadamia, Macadamia jansenii. By benchmarking different methods for long read sequencing, this preprint publication on BioRxiv is a useful, up-to-date comparison of costs and software options for assembly and polishing.

GIH contribution

Valentine Murigneux
Valentine Murigneux

As a successful outcome of the GIH funded project "Assembly of complex genome (Macadamia) using single molecule sequencing", Dual GIH and QCIF team member Valentine Murigneux performed Illumina, Oxford Nanopore Technology (ONT) and PacBio assemblies and assembly evaluation.

Valentine also wrote the manuscript draft with input from all authors.

Dr Subash Rai
Dr Subash Rai

GIH team member Dr Subash Rai performed the ONT library preparation and sequencing.