Published on: 20 September 2021
Through the collaborative project “Dual guide screening” and in association with QFAGE and Agilent, we have generated a workflow for high efficient CRISPR knockout using optimised multiple guide RNA design. This workflow was utilised by GIH team member Dr Di Xia for the research within this publication by Meredith A. Redd, et al, where Di prepared the method for the genome editing strategy design, supervised the CRISPR transfection, troubleshooting and analysis to generate the Asic1a knock-out iPSCs, along with training members of the QFAGE in the procedure.