publication

As a successful follow-on from the GIH funded project "Spatial genomics technologies to study cancer and genetic diseases in tissue contexts", contributing author and team members Stacey Andersen, and Dr Sohye Yoon assisted with experimental set up including library preparation and sequencing on the NextSeq500 (Illumina) instrument for data utilised in this publication.

The M. jansenii genome has been used as a model for testing sequencing methods using a wide range of long read sequencing techniques. Here, we report a chromosome level genome assembly, generated using a combination of Pacific Biosciences sequencing and Hi-C, comprising 14 pseudo-molecules, with a N50 of 52 Mb and a total genome assembly size of 758 Mb of which 56% is repetitive.

As one of the sucessful outcomes from the GIH funded collaborative project Simultaneous identification of RNA-chromatin interactions and transcriptomes in single cells, contributing author and team member of both GIH and the UQ Sequencing Facilty, Stacey Andersen, performed sequencing on the NextSeq of the Illumina libraries and reviewed the manuscript.

Acknowledgment: The Authors noted that the work was supported by funding from the Genome Innovation Hub.
They are part of the GIH collaborative project "Simultaneous identification of RNA-chromatin interactions and transcriptomes in single cells, January 2020"

Through the collaborative project “Dual guide screening” and in association with QFAGE and Agilent, we have generated a workflow for high efficient CRISPR knockout using optimised multiple guide RNA design. This workflow was utilised by GIH team member Dr Di Xia for the research within this publication by Meredith A. Redd, et al, where Di prepared the method for the genome editing strategy design, supervised the CRISPR transfection, troubleshooting and analysis to generate the Asic1a knock-out iPSCs, along with training members of the QFAGE in the procedure.

Acknowledgement: The authors thanked the University of Queensland Genome Innovation Hub for continuing support. They are part of the GIH collaborative project "Simultaneous identification of RNA-chromatin interactions and transcriptomes in single cells, January 2020"

Acknowledgment: The Authors noted that the work was supported by funding from the Genome Innovation Hub external project grants. They are part of the GIH collaborative project "Spatial genomics technologies to study cancer and genetic diseases in tissue contexts,
January 2019".